Composite

Part:BBa_K1025006:Design

Designed by: Weifan Liang   Group: iGEM13_Tsinghua-E   (2013-09-14)


Bitter Defender Part （RBS_B0030）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 618
    Illegal NgoMIV site found at 693
    Illegal NgoMIV site found at 723
    Illegal NgoMIV site found at 943
    Illegal NgoMIV site found at 1061
    Illegal NgoMIV site found at 1328
    Illegal NgoMIV site found at 1523
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1750


Design Notes

The genes were cloned easily by PCR and connected to plasmid backbone by in-fusion cloning.


Source

tnaC and Rho were sythesized by DNA 2.0 corporation according to reference[1].TetA was cloned from plasmid pCM110. [1]Gong, F. & Yanofsky, C. Instruction of translating ribosome by nascent peptide. Science297, 1864-1867, doi:10.1126/science.1073997 (2002).

References